Documents Toxicology Letters 6 results

"The Globally Harmonised System (GHS) for classification and labelling will provide an internationally agreed system for hazard classification of chemical products and for communication of those hazards. Under the system, chemicals will be classified according to their physical (e.g. flammability), health/toxicological and environmental hazards. The toxicological endpoints used in the system are acute toxicity, irritation or corrosivity, sensitisation, carcinogenicity, mutagenicity, reproductive toxicity and chronic or repeat dose toxicity. The intended target audiences are those concerned with transport of dangerous goods, consumers, workers and emergency responders. Labels and safety data sheets (SDS) are the core tools of the GHS hazard communication system, and the harmonised labelling elements are symbols (within a pictogram), signal words and hazard statements. The GHS will use a building block approach in which application may vary according to the circumstances, type of product, and stage of life cycle, allowing selection of the elements appropriate to the needs of the various end users."

"Occupational health regulations and practices in EU Member States have been directed, to a large extent, by the principle of social protection, aiming to protect both the health and the employment of every (candidate) worker. Medical and biomonitoring practices have played a major role in identifying health problems at work and also in establishing preventive measures. Lately, a new tendency is occurring that reduces health protection to compliance with a limited set of standards for workplaces and individuals. According to this approach, 'predictive' medical tests might be used for the selection of the fittest workers. At a time when the labor market is evolving towards less stable forms of work, such a 'standardization approach' may lead to an unequal occupational health policy: the better off being more protected, the more exposed being less protected and the more susceptible being excluded. Instead, biomonitoring tools should only be developed as part of medical surveillance and used for those who need it most."

"This study was undertaken to investigate the effects of genetic polymorphisms of the cytochrome P450 1A1 (CYP1A1) and 2E1 (CYP2E1), and glutathione S-transferases mu (GSTM1) and theta (GSTT1) on urinary 1-hydroxypyrene and 2-naphthol levels, and to estimate the level of exposure to polycyclic aromatic hydrocarbons (PAHs) in aircraft maintenance workers. In 218 Korean aircraft maintenance workers, the geometric means of urinary 1-hydroxypyrene and 2-naphthol were 0.32 and 3.25 micromol/mol creatinine, respectively. These urinary concentrations were approximately at the upper limit of the general population. Mean urinary 2-naphthol concentrations were significantly different between smokers and non-smokers. CYP1A1 and GSTM1 were statistically significant in analyses on both 1-hydroxypyrene and 2-naphthol levels among smokers. The results suggest that smoking has more profound effects on urinary PAH metabolites than does genetic polymorphisms in this population, and that CYP1A1 and GSTM1 activity might be related to the metabolism of 1-hydroxypyrene and 2-naphthol."

"We have analysed liver angiosarcomas from individuals having been occupationally exposed to vinyl chloride (VC) to identify, in cancer-related genes, lesions which could be VC-specific. Two genetic alterations have been identified: the first one is a GGC --> GAT (Gly --> Asp, Asp13p21) mutation at codon 13 in the Ki-ras gene, found in five out of six tumors. The second one is an AT --> TA transversion in the p53 gene resulting in missense mutations at different codons and was found in three out of six tumors. By analysing both the tumors and sera from the same patients, we have shown that the Asp13p21 and mutant p53 proteins could be detected reliably in the serum. We thereafter analysed 225 serum samples, selected from a cohort of about 900 VC-exposed workers, for the presence of the two mutant proteins and p53 antibodies. A statistical analysis supports a strong dose-response relationship between the serum markers positivity and the VC-exposure. A follow-up of this cohort should now allow us to assert the predictive value of these markers."

"Xenobiotic metabolizing enzymes, especially CYP1A1 and GSTM1, are involved in the activation and conjugation of PAHs and are controlled by polymorphic genes. PAHs released from diesel emissions in many cities of the world, especially in developing countries, contribute significantly to the toxic effects of airborne inhalable particles. We have evaluated the gene-environment interaction in Santiago of Chile, studying the contribution of CYP1A1 and GSTM1 polymorphisms on 1-OH-P urinary levels used as the PAHs exposure biomarker. The study was performed on 59 diesel exposed (38 diesel revision workers and 21 subjects working in an urban area as established street vendors) and 44 non-exposed subjects living in a rural area. The 1-OH-P urinary levels of the urban (P=0.043) and rural (P=0.040) populations showed, without considering the genotypes, significant differences between smokers and non-smokers, but no significant differences were found between smokers and non-smokers among the diesel plant workers (P=0.33). Non-smoking subjects of the diesel plants and the urban area showed similar 1-OHP levels (P=0.466) which were significantly higher than those of the subjects living in the rural area (P<0.05). ..."

"This review aims to give an overview of some of the biomarkers that have been used for the monitoring of human exposure to xenobiotics as well as to provide a summary of some of the recent epidemiological studies on male reproductive health of exposure to environmental and occupational toxicants. Possible molecular mechanisms on seminal quality change are also suggested. Studies using various biomarkers have no doubt enabled us to better characterize the effect of environmental pollutants on the male reproductive system. However, the sensitivity and specificity of these biomarkers have not been comprehensively validated. Furthermore, many epidemiological findings are difficult to replicate owing to the inherited methodological problems of male reproductive health investigations, such as the small number of study subjects, low compliance rate, substantial intra-individual variability in semen parameters, measurement techniques and misclassifications based on single assay. Oxidative damage, in particular DNA-damage caused by free radicals, generated either by xenobiotics, or endogenously, is now thought to be a key molecular mechanism associated with semen quality and sperm function. Laboratory studies and epidemiological findings have suggested that the male reproductive system is susceptible to reactive oxygen species (ROS). On the other hand, there is so far no single all-encompassing biomarker of reproductive capacity in men. A panel of biomarkers with specific goals should be considered. Collaborative multidisciplinary studies are also needed to overcome some of the issues mentioned here."